Welcome to AmpliSAT (Amplicon Sequencing Analysis Tools)
14/09/2018 Server was updated and it is running correctly again.
25/06/2018 AmpliCDR3 accepts now as input two paired-end read files and URLs/links to FASTQ files from repositories.
25/06/2018 Improved documentation for AmpliTCR and AmpliCDR3 tools.
04/05/2018 AmpliCDR3 has been optimized for alpha, beta, gamma and delta chains in human and mouse.
04/05/2018 V-J gene usage statistics included into AmpliCDR3 automatic report for human and mouse.
AmpliSAT are a set of online tools that make easy the analysis of Amplicon Sequencing experiments.
Amplicon Sequencing (AS) is a useful technique in the genotyping of complex gene families, such as MHC, with multiple loci and copy number variation among individuals (Babik et al. 2010; Lighten et al. 2014).
AS is widely used for taxonomic classification by amplifying a variety of marker genes: small subunit rRNA (16/18S SSU), large subunit rRNA (23/25/28S LSU), internal transcribed spacers (ITS/ITS1,ITS2), cytochrome c oxidase subunit 1 (CO1) or plant specific genes (rbcL, matK and trnH-psbA) (Sogin et al. 2006; Kress et al. 2014; Joly et al. 2014).
Another novel and promising application of AS is the study of antibody and T cell receptor (TCR) repertoires in mammals and birds (Baum et al. 2012; Georgiou et al. 2014; Ruggiero et al. 2015; Robinson 2015).