Run AmpliCHECK

Run name:
Email (optional):
Sequence file: FASTA/FASTQ (compressed or uncompressed) without spaces, parenthesis or other special symbols in the name
If reads have been already de-multiplexed into separate files (one file per sample), you can pack them into a single .zip or .tar.gz format file and use it as input. In such case you should leave empty the amplicon data section.
Max. 500 MB Download example
Technology: Auto Illumina 454/IonTorrent      Analysis parameters will be optimized for the selected sequencing technology, they can be modified in "Advanced options".

If transcriptomic data is being analyzed or many co-amplifying alleles are expected (>10, e.g. MHC class I), the 'minimum per amplicon frequency' threshold in 'Advanced program parameters' section should be manually adjusted.
Expand results: If selected this option, the results will be printed in 3 spreadsheets (depths, frequencies and errors).
Amplicon data: It is very important to specify all the primer and tag sequences in 5'->3' sense.
Shortening primer sequences to 7-9 nts can increase the number of retrieved sequences (eg. GAGTGTCAT instead of GAGTGTCATTTCTCCAACGGGA).

or file: Max. 100 KB See example

Alleles file (optional): FASTA format Max. 2 MB See example

Advanced program parameters


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