AmpliCLEAN - Amplicon Sequencing CLEANing tool
AmpliCLEAN filters reads not belonging to any amplicon and removes low quality and/or anomalous short/long ones.
Sequenced data may contain reads from other experiments (sequenced together, usually to decrease costs), and low quality or abnormally short/long ones that it is recommended to remove before continuing further analysis to save disk space and time. For this purpose, AmpliCLEAN will separate experiment-specific reads complying the desired length and quality parameters.
AmpliCLEAN takes as input::
- SEQUENCE FILE: FASTQ or FASTA format file (compressed or uncompressed). Multiple sequence files should be packed into a unique .ZIP or .TAR.GZ file.
- AMPLICON DATA *: primer and tag information in a CSV format file as explained in the documentation.
Results can be downloaded on the same page or from an email message after analysis completion.
Output file will be in FASTA or FASTQ (depending of the input file) compressed format, ready to be used in other AmpliSAT tools like AmpliCHECK or AmpliSAS.
For more information, read the documentation.