AmpliLEGACY - Amplicon Sequencing LEGACY genotyping methods
AmpliLEGACY emulates previously published methods for genotyping amplicon sequencing data.
Three genotyping protocols (Sommer et al. 2013; Radwan & Herdegen et al. 2014; Lighten et al. 2014) have been automated in this tool.
Though we encourage the use of AmpliSAS because it has already showed excellent results in error correction and genotyping of Illumina, 454 and Ion Torrent data (Sebastian et al. 2015), these alternative genotyping strategies are equally valid if they are used with the same NGS technology and experimental requirements specified in their original articles.
AmpliLEGACY takes as input 1:
- SEQUENCE FILE 2: reads from the experiment in a FASTQ or FASTA format file (compressed or uncompressed).
- AMPLICON DATA: primer and tag information in a CSV format file as explained in the documentation.
2 This tool CANNOT use as input multiple sequence files packed into a unique .ZIP or .TAR.GZ file.
Results can be downloaded on the same page or from an email message after analysis completion with a similar format than AmpliSAS ones.
For more information, read the documentation.